There are 2 main steps to use MetaGSCA.
First step is for user to upload a gene file or search for predefined Cancer pathways-gene lists on the tool page.
Second step requires selection of one model (Inverse or GLMM), selection of one meta model (Fixed or Random), selection of number of permutation and bootstrap and finally upload of gene expression datasets for analysis.
The add another dataset button is used to add up to 20 different datasets for meta analysis. An EMAIL address is required to get results (gz format) due to long runtime.
The examples provided (genelist and gene expression files) are for the glutamine pathway only.